Thermo Scientific Pierce CT(PEG)12 or carboxy-PEG12-thiol is a methyl- and thiol-terminated compound. Contains a 12-unit polyethylene glycol (PEG) spacer that can be used to modify surfaces (such as quantum dots, monolayers, and magnetic particles).

CT(PEG)12 is a monodentate thiol capped and carboxylic acid PEGylation reagent. PEG compounds have defined molecular weights and spacer lengths and are suitable for modifying surfaces such as quantum dots, self-assembled monolayers, and magnetic particles. Functionalization of solid surfaces with this polyethylene glycol reagent provides carboxylate handles for protein immobilization via EDC cross-linking and significantly reduces nonspecific protein binding.

Characteristics of carboxy-PEG12-thiol:

•Metal binding—The terminal monodentate thiol spontaneously reacts with the gold surface through coordination binding
• Carboxylate—Terminal carboxylic acid groups can be coupled to amine-containing affinity ligands via carbodiimide (EDC) and Sulfo-NHS cross-linking chemistries
• Polyethylene Diol—PEG groups are flexible, non-immunogenic, hydrophilic and significantly reduce non-specific binding to protein method surfaces
• Spacer Arm—12-unit The PEG spacer length is nearly 48 Angstroms, minimizing steric hindrance for protein immobilization and binding assays

Use in surface modificationsMT(PEG)4 and CT(PEG)12 can form hydrophilic "moss" of methyl ether-terminated PEG and PEG containing periodically exposed carboxylic acids. The exposed carboxylic acid groups can be coupled to affinity ligands via a carbodiimide coupling reaction of EDC and sulfo-NHS.

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