SYTOX® Green nucleic acid dye is an excellent green fluorescent nuclear and chromosomal counterstain that is impermeable to living cells, thus Can be used as an effective indicator of dead cells in a cell population.

Also available as a room temperature stable, ready-to-use solution: NucGreen Dead 488 Ready Probes Reagent< /a>.
View other Ready Probes ready-to-use imaging reagents and accessories›

• Impermeable to living cells
• Excitation/Emission: 504/ 523 nm
• Use with 488 nm argon ion laser
• Suitable for mammalian cells and Gram-positive and Gram-negative bacteria
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Easy Viability Measurement
SYTOX® Green can quickly measure cell viability using a flow cytometer, fluorescence microscope, fluorometer or fluorescence microplate reader. It does not penetrate intact cell membranes, but it can easily penetrate damaged cell membranes, which is a characteristic of dead cells. Since fluorescence is enhanced >500-fold when bound to nucleic acids, brief incubation with dead cells using SYTOX® Green produces bright green fluorescence when excited by a 488 nm argon ion laser or any other 450–490 nm light source , the emission peak is 523 nm. The signal produced by dead cells is very bright, so SYTOX® Green is particularly suitable for determining the viability of not only mammalian cells, but also Gram-positive and Gram-negative bacteria.

Invitrogen Series Cell Viability Dyes and Assays
SYTOX® stains for dead cells are available in a variety of colors, including red, blue and orange. Additionally, Invitrogen uses SYTOX® stains for a variety of apoptosis, cell viability and metabolism assays.

For scientific research use only. Not intended for any animal or human therapeutic or diagnostic use.

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• Learn more about all SYTOX® products.