The reagents in the Qtracker™ 565 Cell Labeling Kit use custom targeting peptides to Yellow-green fluorescent Qdot™ 565 nanocrystals are delivered into the cytoplasm of living cells. The Qtracker™ Cell Labeling Kit is designed to label cells growing in culture using high-intensity fluorescent Qdot™ nanocrystals. Once inside a cell, Qtracker™ labels provide strong and stable fluorescence that can be tracked over generations of cells without transferring to neighboring cells in the cell population.

Need a different emission spectrum or longer tracking? View our other Mammalian Cell Tracking Products.

Important Properties:

•The excitation/emission wavelength of the Qtracker™ 565 marker is (405-525/565) nm
• Dedicated to labeling cells grown in culture with high-intensity fluorescent Qdot™ nanocrystals
• Provides strong and stable fluorescence that can be traced over generations of cells< /b>
• Available in eight colors—525 nm, 565 nm, 585 nm, 605 nm, 625 nm, 655 nm, 705 nm or 800 nm emission
• < b>Particularly suitable for long-term tracking or imaging studies of living cells, including cell migration, movement, morphology and other cell function tests

Qtracker™ Cell Labeling Kit uses customized targeting peptides , Qdot™ nanocrystals can be delivered into the cytoplasm of living cells. Cytoplasmic delivery of this mechanism is not specifically enzyme-mediated; therefore, no cell type specificity is observed. Delivery is usually completed in less than 1 hour.

Qtracker™ Cell Labeling Kit’s Qdot™ nanocrystals are compatible with serum-sensitive cells; remain robust under complex cellular environments and a variety of biological conditions, including changes in intracellular pH, temperature, and metabolic activity Fluorescence. In addition, using the Qtracker™ 655, 705 or 800 kits, you can avoid autofluorescence commonly observed in cells or tissues.

Persistent Targeting Signal
Use the Qtracker™ Labeling Kit to observe labeled cells using broad, continuous illumination without the photobleaching typically associated with organic dyes degradation problem. Qtracker™ markers are distributed in cytoplasmic vesicles and are inherited by daughter cells for at least six generations. Fluorescence from Qtracker™ markers is still visible in some cell lines one week after delivery. The Qtracker™ Cell Labeling Kit, which maintains fluorescence within cells for long periods of time, is ideal for studying cell movement, migration, differentiation, morphology, and many other cellular functions. Qtracker™ markers do not leak out of intact cells and are taken up by neighboring cells within a cell population.

Signals can be monitored on a variety of platforms
Live cells labeled with Qtracker™ reagents can be easily monitored on a variety of platforms, including flow cytometry, fluorescence/confocal Microscopes, fluorescence microplate readers, and high-content imaging systems.

Minimal impact on living cells
The cytotoxicity of the materials used in the Qtracker™ Cell Labeling Kit has been tested in a variety of cell lines, including CHO, HeLa, U -118, 3T3, HUVEC and Jurkat cells. Labeling with the Qtracker™ Cell Labeling Kit has minimal impact on the expression of cell surface markers, cell proliferation, cell enzyme activity and cell mobility.

Can be used in a variety of cell tracking studies
Researchers have demonstrated a wide range of applications for Qtracker™ labeled cells, including cell co-culture and cell assembly into heterotypic complexes , multi-lineage differentiation, transdifferentiation and cell fusion, embryonic and mesenchymal stem cell tracking and cell migration dynamics.