Thermo Scientific Pierce SPDP is a short-chain cross-linker that works through NHS ester and pyridyl disulfide reactive groups. The group forms a cleavable (reducible) disulfide bond with the cysteine thiol group to realize the coupling of the amine and the thiol group.

Features of SPDP:

•Reactive groups: NHS Ester&Pyridyl disulfide
•Can react with the following groups:Amino and thiol groups
•When reacting with free thiol groups Releases detectable by-products; reactions can be easily followed by measuring the release of 2-mercaptopyridine at 343 nm
• Disulfide bonds in the spacer can be easily cleaved using 10-50 mM DTT or TCEP (pH 8.5)
•The spacer arm can also be easily cleaved using reducing SDS-PAGE loading buffer
•Cleatable cross-linker enables separation of cross-linked products
•Insoluble in water (first dissolve in DMF or DMSO)
•SPDP cross-linker is membrane permeable and cross-links within cells
•Comparable to many other SPDP-type reagents, including pegylated forms

< b>Applications:
• Reversible cross-linking of proteins
• Addition of sulfhydryl groups to proteins by attachment to lysine residues
• Formation of antibodies through two-step reaction protein cross-linking - enzymes and others Bioconjugates
•Hapten linked to carrier protein for antibody production
•Preparation of immunotoxin conjugates

Specifications:
We follow the higher SPDP is manufactured to specifications to produce highly specific bioconjugates, ensuring data integrity and providing a high degree of consistency. Each batch of SPDP is tested by quantitative NMR (the higher standard for cross-linker purity) to ensure purity >95%.

We offer three SPDP analogues: the standard form (SPDP), a longer spacer derivative (LC-SPDP) and a sulfonated water-soluble variant (Sulfo-LC-SPDP). SPDP( N-succinimide 3-(2-pyridyldithio)-propionate) and LC-SPDP (succinimide 6-(3-[2-pyridyl Dithio]-propionylamido)caproate) is a heterobifunctional, thiol-cleavable and membrane-permeable cross-linker. They each contain an amine-reactiveN-hydroxysuccinimide (NHS) ester that reacts with a lysine residue to form a stable amide bond. The other end of the spacer arm is terminated with a pyridine disulfide group, which reacts with a sulfhydryl group to form a reversible disulfide bond.

Product Reference:
Crosslinking Agent Application Guide -- Search the latest references for this product

Related Products
Pierce™ Premium SPDP
LC-SPDP (succinimide 6-(3-(2-pyridyldithio)propionamido)caproate)