Thermo Scientific Mem-PER Plus Membrane Protein Extraction Kit enables integration of membrane and membrane-associated proteins through a simple reagent-based procedure Small-scale solubilization and enrichment.

Mem-PER Plus Kit Features:

•Extraction and Separation—Extremely low cross-contamination rate (usually less than 10%), membrane protein fractions Only minimal amounts of cytosolic proteins are incorporated into
•Cells or Tissues—Efficient extraction from cultured mammalian cells and mammalian tissues
•Downstream Compatibility —Analyze membrane protein extracts by SDS-PAGE, Western blotting, immunoprecipitation, and protein detection
•Quick and easy—Isolate membrane proteins in about 1 hour
•No special equipment required—only a benchtop microcentrifuge, tubes, homogenizer and pipette are required

Mem-PER Plus kits can be prepared from Efficient isolation of membrane proteins from cultured cells and tissues. It effectively solubilizes integral membrane proteins containing one or two transmembrane domains and separates them from cytosolic proteins. This kit can extract and selectively enrich integral membrane proteins and annexins from cultured mammalian cells and hard and soft mammalian tissues. The Mem-PER Plus kit offers many advantages over the original Mem-PER kit. The protocol is simpler and the components are directly compatible with many downstream applications such as SDS-PAGE, Western blotting, BCA, immunoprecipitation, and amine-reactive protein labeling techniques.

Includes:
The kit contains cell washing solution, permeabilization buffer and solubilization buffer.

Traditional membrane protein separation methods are cumbersome and time-consuming, and require gradient separation and expensive ultracentrifugation equipment. The Mem-PER Plus Membrane Protein Extraction Kit utilizes a selective extraction protocol to enrich integral membrane proteins and annexins from cultured mammalian cells or tissues using reagents based on mild detergents. Traditional separation methods usually require separation based on hydrophobicity, while selective detergent extraction solutions can eliminate this tedious phase separation process, thereby improving experimental reproducibility and processing throughput.

First use a mild detergent to permeabilize the cells to release soluble cytosolic proteins, and then use a second detergent to dissolve the membrane proteins. Extraction efficiency and yield depend on the cell type and the number of times the integral membrane protein has crossed the lipid bilayer. For membrane proteins containing one or two transmembrane domains, extraction efficiencies typically reach 90%. Very few cytosolic proteins are incorporated into the membrane protein fraction, so cross-contamination rates are typically less than 10%.

More product data
•Efficient mammalian membrane protein extraction

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