DAPI is a commonly used nuclear counterstain for fixed cells that emits blue fluorescence after binding to DNA. With NucBlue™ Fixed Cell ReadyProbes™ reagents, we've formulated a room-temperature stable solution of this classic stain in a highly pure form, packaged in a convenient dropper bottle. Just two drops per milliliter are enough to stain cells.

Also: DAPI (4',6-diamidino-2-phenylindole dihydrochloride) aqueous solution.

• No dilution, weighing or pipetting required
• Dropper bottle for easy access – just add 2 drops per ml
• Room temperature stable – can be placed within the field of view or in the cell culture area , easy to use • DAPI can be excited by UV light and can be detected through blue/cyan filters

View other ReadyProbes™ cell staining reagents
View other nuclear stains for imaging
< br>Cell Imaging Applications
DAPI is a classic fluorescent dye that is widely used for nuclear staining of fixed cells. Under fluorescence microscopy, DAPI is excited by UV light and can be detected through a blue/cyan filter (Figure 1).

Usage Recommendations
In most cases, adding 2 drops of NucBlue™ Fixed Cell Stain per ml and incubating for 15 to 30 minutes will produce bright nuclear staining; However, for certain cell types, conditions and applications, optimization may be required. In this case, simply increase or decrease the number of drops until you obtain the optimal stain intensity.
•After binding to DNA, NucBlue™ Fixed Cell Stain can be excited by UV light at 360 nm, with a maximum emission wavelength of 460 nm. Detection can be performed with blue/cyan filters such as DAPI filters, blue GFP filters, or the Semrock BrightLine™ Alexa Fluor™ 350 Dye Filter Kit.
•As the blue nuclear stain of choice for fixed cell imaging experiments, NucBlue™ Fixed Cell Stain is ideal for antibody applications.