Fixable Viability Dye eFluor™ 506 is a viability dye that irreversibly labels dead cells prior to cryopreservation, fixation, and/or permeabilization procedures. Unlike 7-AAD and propidium iodide, cells labeled with Fixable Viability Dye can be washed, fixed, permeabilized, and stained for intracellular antigens without loss of staining intensity for dead cells. Thus, when studying intracellular targets, using Fixable Viability Dye allows the exclusion of dead cells from analysis. Fixable Viability Dye can be used to label cells from all species.

Fixable Viability Dye eFluor™ 506 is excited by the violet (405 nm) laser line, has an emission peak at 506 nm, and can be detected using a 510/50 bandpass filter (equivalent to AmCyan). Please ensure that your instrument is capable of detecting this dye. For compensation, it is recommended to use a sample of target cells stained with Fixable Viability Dye. If the percentage of dead cells is expected to be less than 5%, it is recommended to remove a small cell aliquot and heat it at 65°C for 1 minute, followed by immediate placement on ice for 1 minute. Following this treatment, the heat-killed cells can be mixed 1:1 with live cells and stained with Fixable Viability Dye.

Fixable Viability Dye eFluor™ 506 is supplied as a pre-diluted solution prepared in high quality anhydrous DMSO. Protect from light and moisture when stored. Store at or below -70°C with desiccant. Can be frozen and thawed up to 20 times. Equilibrate vial to room temperature before opening.

Reported Applications
Flow Cytometry