Thermo Scientific Pierce Gaussia-Firefly Luciferase Dual Assay Kit provides simultaneous detection of mammals Essential reagents for intracellularGaussia and red firefly luciferase activity in whole-cell lysates.

Gaussia- Firefly Luciferase Dual Detection Kit Features:

•Simultaneously—Simultaneous detection of two luciferase activities Perform filter-based wavelength separation detection
• Sensitive—Measure bright blue Gaussia and red firefly luciferase activity in the same sample
• Fast—No quenching step required, unlike traditional sequential dual assays
• Multiplex—Can quantify two cell activities in the same sample
< br>Pierce Gaussia-Firefly Luciferase Dual Assay Kit is a highly sensitive assay that supports simultaneous detection of Gaussia and red firefly luciferase activities. Gaussia luciferase served as an experimental reporter gene, with constitutively activated red firefly as a normalization control. As shown in the animated video below, this reporter control panel can simultaneously monitor the activity of experimental reporter genes and control luciferase in a single reading assay without the need for two-step addition of substrate reagents or quenching. The assay working solution contains two luciferase substrates and reacts with simultaneous flash-type kinetics. The resulting luminescence signal can be spectrally resolved using filters. In a single sample, researchers can measure the transcriptional activity of regulatory elements, signal transduction pathways, and the effects of activators or inhibitors.

Product Components:
Cell lysis buffer, reaction buffer, and substrates Vargulin and D-luciferin

Required:< /b>
Sea Firefly and red firefly luciferase reporters; filter sets range from 425-525nm (for Sea Firefly Luc) and 615- 675nm (for red firefly Luc). Photometer or other instrument capable of monitoring luminescence (such as Thermo Scientific Luminoskan Ascent and Varioskan flash microplate readers); injector required to evaluate more than 24 wells at a time.

Applications:
• Study two regulatory elements simultaneously
• Monitor two signaling pathways simultaneously
• Study multiple targets per screen (e.g. off-target Effect)

Application video:
Gaussia luciferase can produce more energy than firefly and natural Renilla luciferase Brighter bioluminescence signal. The bioluminescent signal (λmax= 470nm) produced by Gaussia luciferase is caused by the oxidation of coelenterazine. This reaction does not require adenosine triphosphate (ATP) or other cofactors. Red firefly luciferase is a mutant form of the Japanese firefly luciferase derived from Genji. This luciferase produces a red-shifted radiant light (λ max = 613 nm) caused by the oxidation of D-luciferin in the presence of ATP. Red firefly andGaussialuciferase were used in dual assays because the light output can be spectrally resolved.

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