Thermo Scientific EZ-Link Sulfo-NHS-Biotin is a short-chain, water-soluble biotinylation reagent for Label antibodies, proteins, and other molecules with primary amines.

EZ-Link Sulfo-NHS-Biotin Features:

•Protein Labeling—Biotinylated antibodies facilitate the use of streptavidin resin or probes Needles for fixation, purification, or detection
•Cell surface markers—Only biotinylate surface proteins of whole cells because negatively charged reagents do not penetrate cell membranes
• Amine reactivity—Reacts with primary amines (-NH2) such as lysine side chains or amino termini of polypeptides
• Soluble—Compatible with common NHS ester compounds The more charged sulfo-NHS group improves the water solubility of the reagent
• Irreversible—forms a permanent amide bond; the spacer arm is not cleavable
• Very short —The spacer arm (total length added to the target) is 13.5 Angstroms and contains only native biotin valerate groups.

Sulfo-NHS-Biotin is the shortest of three very similar EZ-Link reagents that are water-soluble, non-cleavable, and work in solution with antibodies, proteins, and any other Simple and efficient biotinylation of primary amine macromolecules. Specific labeling of cell surface proteins is another common application for these unique water-soluble, membrane-impermeable reagents. Different only in spacer arm length, the three Sulfo-NHS ester reagents can optimize labeling and detection experiments in which steric hindrance of biotin binding is an important factor. Sulfo-NHS-Biotin is available in various packaging sizes and complete protein labeling kits.

We manufacture biotin reagents to ensure the highest possible level of overall product integrity, consistency and performance for intended research applications.

BiotinN-hydroxysulfosuccinimide (NHS) ester is a widely used biotin chemical reagent type. NHS-activated biotin reacts efficiently with primary amine groups (-NH2) in alkaline buffers to form stable amide bonds. Proteins (such as antibodies) often have several primary amines that can be targeted for labeling, including the side chain of a lysine (K) residue and the N-terminus of each polypeptide.

BiotinThe various NHS ester reagents vary in length, solubility, cell permeability and cleavability. Non-sulfonated NHS-Biotin is cell permeable but must be dissolved in organic solvents such as DMSO or DMF. Sulfo-NHS biotin (and biotin with a PEGylated spacer) can be dissolved directly in water but is not membrane permeable. Various types containing disulfide bonds can be cleaved using reducing agents, thereby separating the biotin groups from the labeled protein.

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EZ-Link™Sulfo-NHS- Biotinylation Kit
EZ-Link™ Mini Sulfo-NHS-Biotinylation Kit