Image-iT Green hypoxia monitoring reagent is a new, fixable fluorescent compound used to measure hypoxia in living cells. hypoxic conditions. Living cells do not fluoresce in an environment with normal oxygen concentrations but fluoresce when oxygen levels are reduced. Image-iT Green Hypoxia Monitoring Reagent maintains fluorescence when cells/tissues return to normoxic levels, resulting in minimal loss of fluorescent signal from cells/tissues.

Image-iT Green Hypoxia Reagent Features include:
• Fluorescence in low-oxygen environments can indicate hypoxia in living cells
• Fixation—maintain when cells/tissues return to normal oxygen levels Fluorescence Signal
•Easy to use—just add to cell culture medium and image

Image-iT Green Hypoxia Monitoring Reagent is a fluorescent compound that is permeable to living cells and emits in environments with low oxygen concentrations Fluorescence. These properties make it a very useful tool for examining cells and tissues in hypoxic conditions. Image-iT Green Hypoxia Monitoring Reagent is a very sensitive oxygen detector. Unlike pimonidazole adducts, which respond only to very low oxygen levels, the Image-iT Green Hypoxia Monitoring Reagent begins to fluoresce when atmospheric oxygen levels fall below 5%. It responds quickly to this environment and continues to fluoresce after oxygen levels return to normal. These properties make Image-iT Green Hypoxia Monitoring Reagent an ideal tool for detecting hypoxic conditions in tumors, 3D cultures, spheroids, neurons, and more.