pAd⁄PL-DEST™ Gateway™ Vector Kit contains Gateway™-adapted ViraPower™ adenoviral expression vector, pAd⁄ PL-DEST™ vectors for simple recombination-based cloning and adenovirus-based transient expression of target genes in dividing and non-dividing mammalian cells. This vector can be used to generate adenovirus containing the target gene, with expression driven by the promoter of choice. Alternatively, vectors can be used to express small RNA molecules from their corresponding promoters.
Advantages
• Efficient and rapid recombinant cloning
• High titer adenovirus stocks can be generated
• Genes can be efficiently delivered in vitro or in vivo In dividing and non-dividing mammalian cells
• Enable genes of interest to be controlled by promoters of choice
• Generate replication-incompetent viruses to enhance the biosafety of the system
• Suitable for use with Qualcomm Quantitative applications
Key features
• Gateway™ technology enables efficient and rapid cloning
• Promoterless vectors allow the gene of interest to be controlled by the promoter of choice
br>• Human Ad5 sequence (ΔE3) and viral inverted terminal repeat (ITR) for packaging expression constructs into virions
• Ampicillin selection marker
Kit Includes
• pAd⁄PL-DEST™ vector
• pAd⁄CMV⁄V5-GW⁄lacZ control plasmid
Research use only. Not for therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.