pcDNA™3.1/V5-His TOPO™ TA Expression Kit enables Taq amplification PCR The product is cloned into a high-level expression vector in one step. With topoisomerase activation of the pcDNA3.1/V5-His-TOPO™ vector, it takes only 5 minutes to ligate PCR products and generate 90% recombinants on the bench.

In addition, this vector includes the following features:

•Has a strong CMV vector that enables high-level constitutive expression.
•Has a C-terminal V5 epitope tag, which can be used to efficiently detect recombinant proteins using anti-V5 antibodies.
• Has a C-terminal polyhistidine (6xHis) sequence for purification using nickel chelating resin and detection with anti-His (C-terminal) antibodies.