SuperBoost™ Tyramide Signal Amplification is the ideal solution for detecting multicolor fluorescence immunohistochemistry (ICC), immunohistochemistry (IHC) and The most sensitive method for low-abundance targets in ISH applications. The SuperBoost kit combines the brightness of AlexaFluor™ dyes with the superior signal amplification of poly-HRP-mediated tyramine labeling reactions, with sensitivity 10-200 times that of standard methods. SuperBoost kits are also 2-10 times more sensitive than conventional tyramine amplification technologies such as TSA™. For superior research results, SuperBoost kits provide clearer results on key metrics that imaging methods cannot provide.

SuperBoost kits are easy to use and can be easily and flexibly adjusted according to standard ICC, IHC or FISH experimental protocols and are suitable for any type of cells or tissues. Cells labeled with the SuperBoost kit are compatible with any type of microscope, producing high-resolution images for multiplex analysis. This specific kit contains AlexaFluor 594 tyramine (excitation/emission wavelengths 591/617 respectively) that can be detected using a standard red/Texas Red™ filter cube. The kit also contains polyHRP-conjugated goat anti-mouse IgG secondary antibody.

SuperBoost kit features include:
• Detect low-level or hard-to-detect targets with exceptional sensitivity via fluorescence imaging
• Simple protocols and assays using standard filters
• Suitable for high-resolution multi-channel images—co-labeled with DAPI, secondary antibodies and other SuperBoost kits
• Requires 1/10-1/100 of the amount of primary antibody required for standard ICC/IHC/ISH experiments

SuperBoost kits are based on a tyramine signal amplification system that uses the catalytic activity of horseradish peroxidase (HRP) to generate high-density labeling of target proteins or nucleic acid sequences in situ. The amount of primary antibody required for a typical ICC/IHC/ISH experiment using the SuperBoost kit is 1/10-1/100 that of a standard ICC/IHC/ISH experiment. SuperBoost kits provide specific signal intensities well above background, allowing protocols to be easily optimized to detect specific signals from samples where high endogenous autofluorescence is observed

Advantages of the SuperBoost Kit

Using Alexa Fluor Tyramine to Enhance Signal: The SuperBoost Kit utilizes Alexa Fluor amides that react with HRP, resulting in bright, photostable Alexa Fluor dye deposits on surrounding proteins and other similar molecules. The SuperBoost kit is the only kit that combines the brightness of Alexa Fluor dyes with the enhanced power of tyramine signal amplification to generate superior signals.

Poly-HRP Advantages: Unlike TSA, the SuperBoost kit uses poly-HRP conjugated secondary antibodies. In this system, multiple HRP enzymes are coupled to short-chain polymers, which enhances the signal several times compared to conventional HRP systems. The structural form of poly-HRP allows the antibody to effectively penetrate cells or tissues like conventional HRP-conjugated secondary antibodies. The average enzyme/antibody protein molar ratio is "4".

Stop Solution: As with any enzyme-based labeling system, signal overflow can occur with this system. The SuperBoost kit contains HRP stop solution to stop the HRP reaction. HRP Stop Solution can be used to obtain the highest possible signal without increasing background signal. Images generated using optimized HRP reaction times are as clear as those produced using standard ICC/IHC/ISH methods, but are 10-200 times more sensitive.

Background Reduction: SuperBoost kits contain blocking reagents to eliminate or reduce endogenous peroxidase and fluorescence background signals. These blocking agents help ensure that only specific signals are enhanced while non-specific/background signals are blocked.