SuperSignal West Atto Super Sensitive Chemiluminescence Substrate is an ultra-sensitive enhanced chemiluminescence (ECL) substrate available For high-Ak level protein detection. SuperSignal West Atto Substrate provides extremely high levels of sensitivity and excellent signal-to-noise ratio compared to other commercially available high-performance horseradish peroxidase (HRP) substrates. –SuperSignal West Atto Supersensitive chemiluminescence substrate properties include:


• Sensitivity: low femtogram level to high attogram level
• Stability: working solution up to 48 at 4°C hours, kit up to one year
• Compatibility: Nitrocellulose and PVDF membranes
• Signal duration: up to six hours
• Recommended primary antibody concentration: 1:1,000– 1:5,000 dilution Dilution (0.2–1.0 μg/mL)
• Recommended secondary antibody concentration: 1:100,000–1:250,000 Dilution (4–10 ng/mL)

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SuperSignal West Atto ultra-sensitive chemiluminescent substrate designed for use in Western blotting Achieve superior sensitivity and signal-to-noise ratio in your analysis for very low-abundance targets or precious samples. When used in conjunction with optimized antibody concentrations and blocking buffers, SuperSignal West Atto substrate is capable of detecting low-abundance target proteins even at levels undetectable using typical high-performance ECL substrates detection.