Gibco™ Sf21 cells are commonly used to isolate and propagate recombinant baculovirus stocks and generate recombinant proteins. Gibco™ Sf21 cells are adapted for serum-free suspension culture in Gibco™ Sf-900™ II SFM, saving significant time and expense associated with culture adaptation. Gibco™ Sf21 cells (frozen in Gibco™ Sf-900-™ II SFM) are characterized by:
• Recombinant protein expression in multiple expression systems
• Grow well in adherent or suspension culture
• Quality and performance testing

Recombinant protein expression in multiple expression systems
UsageBaculoDirect ™ Baculovirus Expression System,< a href="https://products.invitrogen.cn/ivgn/product/K81001">Bac-to-Bac™ Baculovirus Expression System or InsectDirect™ Expression System enables high-level protein expression in Sf21 cells.

Grows well in adherent or suspension cultures
Each vial containing 1 × 10⁷ cells can be thawed directly into suspension or Adherent cultures. For protocols for adherent and suspension growth in Gibco™ Sf-900™ II SFM, see the product manual. To facilitate workflow, cultures can be easily transferred between the two conditions, but we recommend keeping the stock culture in suspension.

Quality and Performance Testing
Each batch of Gibco™ Sf21 cells is tested to ensure cell growth and viability after cryopreservation and recovery.

Product Usage
Research use only. Not for therapeutic or diagnostic use in humans or any animal. NOTE: Please handle any potentially biohazardous materials under at least Level 2 biosafety precautions. This product contains dimethyl sulfoxide (DMSO), a hazardous test material. Please review the Material Safety Data Sheet before operation. Please review the Material Safety Data Sheet before operation.