Thermo Scientific Pierce Sulfo-SMCC No-Weigh specification is a water-soluble amine-to-sulfhydryl cross-linker containing NHS esters and Maleimide reactive groups located at opposite ends of a medium length cyclohexane spacer arm (8.3 Å).

Features of Sulfo-SMCC No-Weigh specifications:

•Reactive groups: sulfo-NHS EstersandMaleimides
•React with the following groups: Amino and thiol groups Group
• Water-soluble (compared to SMCC) and therefore can be performed in physiological solutions Cross-linking
• High-purity crystallization reagents can be used to generate high-purity maleimide activated derivatives
• Cyclohexane bridges provide additional stability to the maleimide groups, making Sulfo-SMCC Ideal cross-linker for maleimide activated proteins. Maleimide groups are stable in 0.1 M sodium phosphate buffer (pH 7) for 64 hours at 4°C.

Sulfosuccinimide-4-(N-maleimidomethyl)cyclohexane-1-carboxylate (Sulfo-SMCC) is a Non-cleavable and membrane-impermeable cross-linker. It contains an amine-reactiveN-hydroxysuccinimide (NHS ester) and thiol-reactive maleimide groups. NHS esters react with primary amines at pH 7-9 to form stable amide bonds. Maleimide reacts with sulfhydryl groups at pH 6.5-7.5 to form stable thioether bonds. Due to the presence of a cyclohexane bridge in the spacer arm, the maleimide group of Sulfo-SMCC and SMCC The pellet is stable at pH values up to 7.5. Because it contains hydrophilic sulfonyl groups, Sulfo-SMCC dissolves up to ∼10 mM in water and many common buffers, thus avoiding the use of organic solvents that may disrupt protein structure.

Applications:
•Abzyme labeling - both enzyme and antibody specificity are preserved
•Create specific bioconjugation through one- or two-step cross-linking reactions< br>•Creation of thiol-reactive, maleimide-activated carrier proteins for conjugation of haptens

Specifications:
Sulfo-SMCC is manufactured to the highest specifications, To produce highly specific bioconjugates and maleimide-activated proteins, ensuring data integrity and providing a high degree of cross-linking consistency. Each batch of Sulfo-SMCC is tested to meet the following minimum quality standards.

• Identification: IR scan shows only peaks with structural and functional group characteristics of Sulfo-SMCC cross-linker
• Purity: ≥90% (quantified by NMR method, higher standard for cross-linker purity )
• Solubility: ≥10 mg/mL (dissolved in deionized water)

Product Reference:
Crosslinking Agent Application Guide--Retrieve the latest version of this product References

Related Products
Pierce™ Premium Sulfo- SMCC (Sulfosuccinimide 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)
SM(PEG)4 (PEGylated SMCC cross-linking agent)