• Comprehensive—Contains preformulated reagents for digestion cleavage, peptide purification columns, and optimized protocols for up to 20 samples
• Optimized—Simplified Protocols and reagents to minimize the steps and time required to process samples
• Flexible—Reagents and protocols have been validated with cell, plasma, and tissue samples in 10-100 µg sample volumes
• Time Saving—Sample processing time reduced from more than one day to less than three hours
• Compatible—Final formulations can be used for direct MS analysis and other downstream applications, including TMT Label

The preparation of peptide samples for MS analysis is complex, with numerous steps and changeable protocols, resulting in differences in the quality and quantity of peptides. This kit is designed to improve reproducibility while saving manual work and processing time. By adding a universal nuclease to reduce viscosity from nucleic acids (alternative to sonication), a fast “one-pot” reduction/alkylation solution for cysteine modification (carbamide methylation, +57.02) and for more A fully digested trypsin/Lys-C protease blend that reduces the number of steps and processing time. Additionally, the kit includes a peptide purification column and buffers for preparing detergent-free peptide samples for MS analysis. Optimized reagents and protocols can produce high-quality peptides that are compatible with TMT labeling and other downstream applications, or ready for MS analysis.