MagMAX Virus/Pathogen Nucleic Acid Isolation Kit is designed to isolate viruses and Gram-negative bacteria from samples such as blood, swabs, RNA and DNA were recovered from urine and virus transport medium (VTM). The kit uses MagMAX magnetic bead technology to ensure reproducible recovery of high-quality nucleic acids that are compatible with a variety of applications, including real-time PCR, digital PCR, and next-generation sequencing.
Features of the MagMAX Virus/Pathogen Nucleic Acid Isolation Kit include:
• Automated pre-made protocols designed for KingFisher Flex and KingFisher Duo Prime systems
• Rapid procedure, ready for processing in <30 minutes 96 samples
•Flexible protocol accommodates sample volume input from 200 μL to 2 mL of biological fluids or transfer media
•No need for carrier RNA
•Elution volumes range from 50 to 100 μL
Larger sample input in 96-well deep well or 24-well deep well plate format
Optimized reagents included in the MagMAX Virus/Pathogen Kit allow for larger sample input volumes, allowing Increase the amount of RNA and/or DNA recovered. Up to 400 μL of sample can be processed per well in KingFisher 96-well deep well plates. For larger sample volumes, KingFisher 24-well deep well plates are available, capable of processing up to 2 mL of sample per well.
Advantages of MagMAX magnetic bead purification
Magnetic beads have many advantages compared to other technologies for isolating nucleic acids. Magnetic beads bind nucleic acids more efficiently than glass fiber filter columns, resulting in higher and more consistent yields. Additionally, since no filter columns and vacuum manifolds are used, there is no risk of cell particles in the sample clogging the filter column. This clogging problem is particularly important for large amounts of protein-rich samples commonly used for virus detection, such as whole blood or plasma.
Dynabeads MyOne Silane in the MagMAX Virus/Pathogen Total Nucleic Acid Isolation Kit has an optimized silica-like surface chemistry and high specific surface area, enabling efficient kinetics and high-sensitivity nucleic acid capture. These properties make Dynabeads MyOne Silane ideal for recovering low concentrations of viral RNA and DNA from body fluids. Once captured, these nucleic acids can be eluted in 50 μL elution buffer for downstream applications.
For Research Use Only. Not for use in diagnostic procedures.