Thermo Scientific GPCR Extraction and Stabilization Reagents efficiently extract and stabilize G-coupled protein receptors from cultured mammalian cells or tissues. (GPCR) and other membrane-associated proteins while retaining longer-term receptor integrity.

GPCR Extraction and Stabilization Reagent features include:
•Ease of use—all-in-one formula extracts and stabilizes GPCRs in one hour
•GPCR Storage —Stabilizes receptors in detergent micelles to ensure receptor function
•Optimized—Formulations and procedures enable gentle and efficient extraction of GPCRs from plasma membranes
•Compatible—lysates are directly compatible with downstream applications including Western blotting, immunoprecipitation, and receptor ligand binding assays

GPCR extraction and stabilization reagents are easy to use. Start by harvesting the sample, washing, The reagent is then used to extract the receptor and the resulting whole cell lysate contains solubilized stable receptor. The reagent has been tested for use with endogenous and overexpressed GPCRs and has been tested. Validation of receptor function by ligand binding assay

GPCRs are a large family of receptors with seven transmembrane domains that play an important role in transmitting signals across cell membranes. They are genetic and immune systems. Effective drug targets for disease and potential biomarkers and therapeutic targets for cancer treatment. A major challenge in GPCR research is the ability to efficiently extract receptors from membranes without compromising the structural and functional integrity of the GPCR. The reagent encapsulates receptors in detergent micelles optimized to ensure receptor functionality for up to one week after extraction when stored at 4°C and up to one week when stored at -20°C month, providing greater flexibility for receptor analysis

References
1. Nieto Gutierrez A, McDonald PH.GPCRs: Emerging anti-cancer drug targets.Cell Signal .2018 Jan;41:65-74.
2.Zhang S, Togo S, Minakata K, Gu T, Ohashi R, Tajima K, Murakami A, Iwakami S, Zhang J, Xie C, Takahashi K. Distinct roles of cholinergic receptors in small cell lung cancer cells.Anticancer Res.2010 Jan;30(1):97-106.