GlycoBlue coprecipitant consists of a blue dye covalently linked to glycogen, a branched chain carbohydrate , mainly used as nucleic acid co-precipitating agent. The attached dye increases the visibility of the particles. This product is an ideal coprecipitant for 1/100 dilution of stock solutions in nuclease protection assay kits.

Features of GlycoBlue co-precipitating agent:
• Suitable for RT-PCR
• Increase particle quality and visibility
• Quantitative recovery of low concentration (ng/mL) nucleic acids
• Prevent Particle Loss in Nuclease Protection Assays

What are coprecipitates?
Co-precipitates are inert substances used to assist in the recovery of nucleic acids during ethanol precipitation. Although they can be used to precipitate large amounts of nucleic acid, they are also essential for the quantitative recovery of small amounts of nucleic acid in dilute solutions. Generally, such molecules are used only to reveal the granular precipitate after centrifugation.

GlycoBlue co-precipitating agent can be added to nucleic acid solutions at a final concentration of 50–150 µg/mL. When typical acetate/alcohol precipitation is completed, GlycoBlue coprecipitant will precipitate nucleic acids, promoting good RNA or DNA recovery while increasing particle size and visibility. Because glycogen does not contain significant amounts of nucleic acid, it is often preferred as a coprecipitate to yeast RNA, particularly in applications where nucleic acid quality is assessed, or where added nucleic acid may interfere with or compete with subsequent enzymatic reactions.

As with most other preparations of this coprecipitant, the glycogen used is isolated from rabbit liver (biological source). Glycogen is first treated with proteinase K and SDS to remove all contaminating nucleases, then phenol/chloroform extraction, ethanol precipitation, and finally resuspended in nuclease-free water. We guarantee that this glycogen is free of RNase and DNase.