Dynabeads MyOne Streptavidin T1 is designed to isolate and process biotinylated nucleic acids, antibodies or other biotin The gold standard for chemical ligands and targets. The extremely high binding affinity of the streptavidin-biotin interaction (K_d=10^-15) has been used in a large number of applications. Advantages and features:

• Can directly and quickly separate any biotinylated molecules
• Flexible experimental protocols, mild and efficient liquid phase reaction kinetics
• Low-charge and neutral magnetic beads, suitable for For binding proteins, peptides and antibodies
• Small, uniform size ensures a large surface area and high capacity for target molecules per mg microbeads
• Low deposition rate but high iron content ensures Fast Magnetic Separations
• Biomagnetic protocols that can be easily and flexibly adapted to automated platforms
• High batch-to-batch reproducibility ensures consistent results across applications

About Dynabeads MyOne Streptavidin T1
These uniform superparamagnetic beads are 1 µm in diameter and feature a single rather than multiple layers of recombinant strepavidin covalently coupled to surface and further blocked with BSA. The monolayer of streptavidin leaves the vast majority of biotin binding sites available for binding not only free biotin but also biotinylated ligands/targets. The magnetic beads exhibit fast liquid-phase reaction kinetics. Its specific, defined surface enables efficient capture, separation and downstream processing. The single layer of streptavidin ensures negligible leakage levels without excess adsorbed streptavidin, ensuring consistent and reproducible results. 1 µm Dynabeads MyOne magnetic beads have large surface area, high capacity, high magnetic attraction efficiency and low deposition rate during culture. It can be customized for automation solutions that require high throughput.

Applications
Streptavidin-conjugated Dynabeads have been used and cited in numerous applications over the past 15 years. Key applications include single-stranded DNA template preparation, isolation of RNA and DNA-binding proteins, immobilization of large DNA fragments, product purification and sequencing, and nucleic acid-specific capture. Easily and flexibly adaptable to automated processes. Dynabeads are used on more than 25,000 routine IVD instruments worldwide.

Binding Capacity
Both molecule size and biotinylation procedure affect binding capacity. Binding capacity also depends on available space and charge interactions between beads and molecules and between molecules. After binding, each avidin molecule on the bead surface has two or three biotin binding sites. Per mg of Dynabeads MyOne Streptavidin T1 typically binds:

•950–1500 pmol free biotin
• ∼20 µg biotinylated IgG
• ∼400 pmol biotinylated Peptides
• ∼20 µg ds-DNA
• ∼400 pmol ss-oligonucleotide