Use Thermo Scientific 10X Buffer Y to ensure optimal reaction conditions for restriction endonucleases, and combine this buffer with BSA pre-mixed for enhanced stability. Our five-buffer system ensures optimal reaction conditions for each restriction enzyme. The system includes 10 XB (blue) buffer, G (green) buffer, O (orange buffer), R (red) buffer, and Tango (yellow) buffer. All restriction enzymes are supplied in color-coded tubes to indicate the recommended reaction buffer. Recommended buffers and/or universal Tango buffers are provided with each enzyme.

Tango buffer is a buffer designed for double digestion of DNA using conventional restriction enzymes. For more information, visit Thermo Scientific DoubleDigest Tools.

To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of buffer will not cause BSA to precipitate.

Thermo Scientific restriction enzymes exhibit 100% certified activity in recommended buffers. However, some enzymes require additives to achieve 100% activity. For example, AjuI, AlfI, BdaI, BplI, BseMII, FaqI, Eco57I, Eco57MI, Hin4I, and TsoI require S-adenosylmethionine (also provided with the enzyme), whereas AarI and BveI require an oligonucleotide (also provided with the enzyme). enzyme provided). Esp3I requires DTT.