Sheared salmon sperm DNA at concentrations of 100–200 μg/mL is an excellent choice in Northern prehybridization and hybridization buffers. Effective blocker.

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This sheared salmon sperm DNA has been treated with Proteinase K to remove any contaminating nucleases before being subjected to phenol:chloroform and ethanol precipitation. extraction. Salmon sperm DNA has been rigorously tested to be free of contaminating non-specific endonuclease, exonuclease and RNase activities, and was reconstituted with nuclease-free water in a 1 mL tube to a concentration of 10 mg/mL (1 mL per tube) solution.