Ambion™ Linear Acrylamide (5 mg/mL) can be used as a nucleic acid co-precipitating agent due to small amounts of contamination from other carriers Nucleic acids can also be amplified, making it ideal for PCR and RT-PCR reactions. It is supplied in five tubes of 1 mL each.

•Ideal for RT-PCR
• Increase pellet quality
• Quantitative recovery of low concentration (ng/mL) nucleic acids
• Avoid pellet losses in nuclease protection assays

What is a coprecipitant?
Co-precipitating agents are inert substances that aid in nucleic acid recovery during ethanol precipitation. Although they can be used to precipitate large amounts of nucleic acid, they are also essential for the quantitative recovery of small amounts of nucleic acid in dilute solutions. Generally, such molecules are used only to reveal the granular precipitate after centrifugation. The advantage of linear acrylamide over other co-precipitating agents is that it is chemically synthesized and is not processed with biologically derived reagents (such as proteases). Therefore, it is not contaminated by biological material, making it ideal for RT-PCR. It has been proven to precipitate picogram-level DNA fragments of 20 base pairs or more without precipitating shorter fragments and free nucleotides. Linear acrylamide can be used to separate reaction products from unincorporated nucleotides and most oligonucleotide primers. It should be used at a final working concentration of 10–20 µg/mL. Linear acrylamide does not interfere with A_260/280 readings.