It is recommended that the Plant RNA Isolation Assistant Kit be used in conjunction with the RNAqueous Kit or the MagMAX Plant RNA Isolation Kit for purification of plant tissues. of total RNA.

•Removes common plant contaminants such as polyphenols and polysaccharides
•No phenol extraction, alcohol precipitation or protease digestion steps required when used with the recommended kit

Plants The RNA Isolation Assist Kit contains polyvinylpyrrolidone (PVP), a high molecular weight polymer that binds to contaminants commonly found in plant tissues, such as polyphenols and polysaccharides. The glass fiber filter column-based separation used in the RNAqueous Kit and MagMAX Plant RNA Isolation Kit is particularly suitable for plant RNA isolation because it does not contain any alcohol precipitation step; many common contaminants in plant tissues are known to be involved in the alcohol precipitation process. co-precipitates with nucleic acids. When used with the RNAqueous Kit or the MagMAX Plant RNA Isolation Kit, the procedure involves disrupting plant tissue in a guanidinium-based lysis buffer with the addition of Plant RNA Isolation Accessory Reagent. Centrifuge briefly and remove contaminants. The supernatant was passed through a glass fiber filter column that bound RNA. The filter column is then washed, and the RNA is eluted in a small volume (50 μL or more) of elution solution.

Auxiliary Products
The Plant RNA Isolation Auxiliary Kit is designed for use with the RNAqueous Kit (Cat. No. AM1912) or the MagMAX Plant RNA Isolation Kit (Cat. No. A33784). RNA purification from plant tissues.