10X FastDigest Green Buffer is a proprietary digestion buffer that supports 100% of all FastDigest restriction enzymes active. DNA/RNA modifying enzymes such as ligases, phosphatases, kinases, and mesophilic DNA polymerases are also 100% active in FastDigest Green Buffer. Therefore, enzymes used in downstream applications can be added directly to the FastDigest reaction mixture

Advantages:
• Perform duplexes and multiplexes in a single buffer using any combination of enzymes Enzyme digestion.
•No need for continuous digestion or buffer replacement.
•The reaction mixture can be loaded directly onto the gel.
• Save pipetting time and eliminate the need for DNA loading dye.