Thermo Fisher 60111-103 Each SMART Digestimmunoaffinity (IA)protein AReagent test kit
$2988.41 / Parcel$3276.76 Min.order:1
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Achieve fast, simple, highly reproducible, and sensitive immunocapture and protein digestion in a single well using the Thermo Scientific™ SMART Digest™ Immunoaffinity (IA) Protein A Kit. These kits are designed to eliminate challenges often encountered during biomarker quantitation by providing an easy-to-use capture digestion workflow that takes only a few hours, not overnight. The SMART Digest IA Protein A Kit enables targeted purification of immunoglobulins due to their strong affinity for binding to the Fc portion of the molecule.

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Addressing the challenge of low-level quantitation in complex matrices

The need to identify low-level biomarkers forces the analytical industry to confront extremely high levels of analyte and sample complexity, making quantitative analysis a challenging task. Challenging work.

Because these proteins are often present at low levels in complex biological matrices, immunoaffinity capture steps are often used to purify samples and increase sensitivity. This step is usually followed by protein digestion. Current processes are often time-consuming, involve multiple steps, and are easily non-reproducible.

Immunocapture and protein digestion in a single well

These IA kits provide a process that is:

  • Fast< /li>
  • Simple
  • Highly repeatable
  • Sensitive
Unique design reduces workflow to hours

In the SMART Digest IA Kit, the immunocapture reagent and temperature-activated thermostable trypsin are co-immobilized on the resin. After enrichment, digestion is accelerated by activating enzymes at high temperature under protein denaturing conditions, allowing the workflow to take hours instead of overnight.