5'

↓

N7

G

A

A

N7

T

T

G

G

A td>

G

N11

↓

3'

3'

↑

N12

C

T

T

N7

A

T td>

A

C

C

N6

↑

5'

Thermo Scientific FastDigest AjuI restriction enzyme recognizes^(7/12)GAA( N)7TTGG(11/6)^ site and cleaves optimally within 5–15 minutes at 37°C using Universal FastDigest Buffer.

Thermo Scientific FastDigest AjuI is one of a series of high-end fast restriction enzymes that are 100% active in both Universal FastDigest and FastDigest Green Reaction Buffers.

Universal buffer can quickly complete DNA single, double or multiple enzyme digestion in 5–15 minutes, without the need to change buffers or subsequent DNA purification steps. For a table of enzymatic activities, heat inactivation, and incubation times for this and other FastDigest restriction enzymes, see Reaction conditions for FastDigest enzyme< /a>. DNA modifying enzymes (such as Klenow fragment, T4 DNA Ligase, alkaline phosphatase and T4 DNA Polymerase) are 100% active in FastDigest buffer. Therefore, enzymes used in downstream applications can be added directly to the FastDigest reaction mixture.

For convenience, FastDigest Green Buffer includes 1 density reagent and 2 tracking dyes for loading digestion reaction products directly onto the gel.

Shorter incubation times and optimized universal FastDigest buffer composition eliminate star activity effects.

Features

•100% activity of all FastDigest enzymes in universal buffers
•100% buffer compatibility with downstream applications
• Complete digestion in 5–15 minutes
• Load directly onto gel
• No star activity
• 176 FastDigest enzymes available

Applications< /b>

•Molecular cloning
•Restriction site mapping
•Genotyping
•Southern blot
•Restriction fragment length polymorphism (RFLP)
• SNP Analysis

Remarks:FastDigest AjuI requires SAM for its activity. Some substrates are difficult to digest completely with FastDigest AjuI. FastDigest AjuI cleaves on both sides of the interrupted recognition site, forming a double-stranded nick. The exact cleavage position depends on the sequences flanking the recognition site and may be shifted by one base pair. For a single sequence, the two cleavage positions will be mixed, with most cleavage occurring at one of the positions.

FastDigest Green Buffer provides the same high performance as colorless FastDigest Buffer in DNA digestion reactions and downstream applications. For applications requiring product analysis by fluorescence excitation (eg determination of concentration under UV light), the use of colorless FastDigest buffer is recommended.

See product specifications for information on methylation sensitivity.