The Thermo Scientific GeneJET Plasmid Midiprep Kit is sufficient for 100 preparations and is designed for purification from recombinant
E. coli i>Designed for large-scale isolation of high-quality plasmid DNA in culture. The kit uses silica membrane technology in a convenient spin column format. Recover up to 200 μg of high-copy-number plasmid DNA per preparation. Purified plasmid DNA can be used in a variety of molecular biology procedures such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and robust cell line transfection.
Product Advantages
•Efficient—obtain up to 200 μg of high-quality plasmid DNA in 50 mL of bacterial culture
•High concentration of purified DNA—> 400 ng/μL
• Convenient—spin column format, centrifugation or vacuum protocol
•Quick< /b>—The procedure takes 60 minutes, much faster than using a gravity flow column
Applications
• Isolated plasmid DNA is suitable for all routine molecular biology procedures , including:
•FastDigest restriction enzymeDigestion
•Automated fluorescence sequencing
•PCR
•Cloning
•Transformation< br>•In VitroTranscription
•Robust Cell Line TransfectionFor Research Use Only. Not for use in diagnostic procedures .