CellLight™ ER-RFP (BacMam 2.0) easily labels the endoplasmic reticulum in living cells using red fluorescent protein (RFP) (ER). You simply add the reagent to the cells, incubate overnight, and start imaging the next morning.

Want to label other cellular structures? Learn more about CellLight™ fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology and expresses fusions to calreticulin and KDEL ( RFP of the ER signal sequence (ER retention signal) with minimal cell damage. You can use fluorescence imaging and multiplexing with other fluorescent proteins or organic dyes to observe ER-RFP behavior in living cells.

Cells expressing CellLight™ constructs can also be formaldehyde fixed for multi-pass imaging using immunocytochemistry techniques.

CellLight™ Technical Features:
•Quick and Convenient: Simply add CellLight™ reagent to cells, incubate overnight, and then image—or freeze and store for subsequent experiments Use
• Efficient: Transduction rates up to 90%, suitable for a variety of mammalian cell lines, including primary cells, stem cells and neurons
• Flexible: b>In multiplex assay experiments or co-localization studies, multiple BacMam reagents can be co-transduced; expression levels can be tightly controlled by simply adjusting the dose
• Low toxicity:CellLight™ reagents will not Replicates in mammalian cells and is suitable for Biosafety Level (BSL) 1 handling

BacMam Technology
CellLight™ ER-RFP (BacMam 2.0) is calreticulin and KDEL The fusion construct of the ER signal sequence (ER retention signal) and TagRFP can accurately and specifically target cellular ER-RFP. This fusion construct, packaged in the insect virus baculovirus, does not replicate in human cells and is designated Biosafety Level (BSL) 1, making it safe for use in most laboratories. BacMam technology transduces/transfects most mammalian cell types with high efficiency and ultra-low toxicity. This transient transfection assay lasts up to five days from overnight incubation—sufficient for most dynamic cell analyses. As with all transfection/transduction techniques, the BacMam method does not transfect/transduce all cells with equal efficiency and is therefore not well suited for cell population studies or automated imaging/counting. CellLight™ reagents are ideal for cellular or subcellular co-localization experiments, as well as studies of cellular function that require exceptional resolution.