CellLight™ Mitochondria-GFP (BacMam 2.0) easily labels mitochondria in living cells using green fluorescent protein (GFP). You simply add the reagent to the cells, incubate overnight, and start imaging the next morning.

Want to label other cellular structures? Learn more about CellLight™ fluorescent protein labeling tools

This ready-to-use construct is transfected into cells using BacMam 2.0 technology, where it expresses a fusion to E1 alpha pyruvate Dehydrogenase leader sequence GFP. You can observe mitochondria-GFP behavior in living cells independent of mitochondrial membrane potential and can be labeled with a variety of tracking and tracing dyes to image dynamic cellular processes.

Cells expressing CellLight™ constructs can also be formaldehyde fixed for multi-pass imaging using immunocytochemistry techniques.

CellLight™ Technical Features:
•Quick and Convenient: Simply add CellLight™ reagent to cells, incubate overnight, and then image—or freeze and store for subsequent experiments Use
• Efficient: Transduction rates up to 90%, suitable for a variety of mammalian cell lines, including primary cells, stem cells and neurons
• Flexible: b>In multiplex assay experiments or co-localization studies, multiple BacMam reagents can be co-transduced; expression levels can be tightly controlled by simply adjusting the dose
• Low toxicity:CellLight™ reagents will not Replicates in mammalian cells and is suitable for Biosafety Level (BSL) 1 handling

BacMam Technology
CellLight™ Mitochondria-GFP (BacMam 2.0) is an E1 alpha pyruvate deoxidized The fusion construct of hydrogenase leader sequence and emGFP can accurately and specifically target cellular mitochondria-GFP. This fusion construct, packaged in the insect virus baculovirus, does not replicate in human cells and is designated Biosafety Level (BSL) 1, making it safe for use in most laboratories. BacMam technology transduces/transfects most mammalian cell types with high efficiency and ultra-low toxicity. This transient transfection assay lasts up to five days from overnight incubation—sufficient for most dynamic cell analyses. As with all transfection/transduction techniques, the BacMam method does not transfect/transduce all cells with equal efficiency and is therefore not well suited for cell population studies or automated imaging/counting. CellLight™ reagents are ideal for cellular or subcellular co-localization experiments, as well as studies of cellular function that require exceptional resolution.