Thermo Fisher D399 100 mg H2DCFDA (H2-DCF, DCF)
$361.76 / Parcel$401.91 Min.order：1
D399 100 mgH2DCFDA (H2-DCF, DCF)
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Cell-permeable 2',7'-dichlorodihydrofluorescein diacetate (H2< /sub>DCFDA) (also known as dichlorofluorescein diacetate) is a chemically reduced form of fluorescein used as an indicator of reactive oxygen species (ROS) in cells, e.g. Generation of reactive oxygen intermediates in neutrophils and macrophages. After cleavage and oxidation of the acetate group by intracellular esterases, the nonfluorescent H2DCFDA is converted into highly fluorescent 2',7'-dichlorofluorescein (DCF). Higher cell retention versions of this indicator are available (C400, C2938).
ROS indicator quality standards:
•Excitation/emission wavelength: ∼492–495/517–527 nm
• Product is air sensitive and should be stored in dry argon or In nitrogen
• The product can be dissolved in DMSO, DMF or ethanol for use
• The indicator is permeable to cells (see the literature for cell loading protocols)
• Flow cytometer, fluorometer, micropore can be used Monitor the fluorescence signal with a plate reader or fluorescence microscope (equipped with an excitation light source and filters suitable for fluorescein)
Find more ROS indicators
We offer A series of Molecular Probes™ products for the generation of reactive oxygen species (ROS) (including singlet oxygen, superoxide, hydroxyl radicals, various peroxides and hydrogen peroxide) and for corresponding fluorescence detection in solutions . For more information about these products, please consult the Molecular Probes™ HandbookSection 18.2—Generating and Detecting Reactive Oxygen.
ROS indicator quality standards:
•Excitation/emission wavelength: ∼492–495/517–527 nm
• Product is air sensitive and should be stored in dry argon or In nitrogen
• The product can be dissolved in DMSO, DMF or ethanol for use
• The indicator is permeable to cells (see the literature for cell loading protocols)
• Flow cytometer, fluorometer, micropore can be used Monitor the fluorescence signal with a plate reader or fluorescence microscope (equipped with an excitation light source and filters suitable for fluorescein)
Find more ROS indicators
We offer A series of Molecular Probes™ products for the generation of reactive oxygen species (ROS) (including singlet oxygen, superoxide, hydroxyl radicals, various peroxides and hydrogen peroxide) and for corresponding fluorescence detection in solutions . For more information about these products, please consult the Molecular Probes™ HandbookSection 18.2—Generating and Detecting Reactive Oxygen.
For Research Use Only. Not for use in diagnostic procedures.
