Thermo Fisher EN0202 500 units RNase H (5 U/μL)
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EN0202 500 unitsRNase H (5 U/μL)

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Thermo Scientific Ribonuclease H (RNase H) specifically degrades RNA strands in RNA-DNA hybrids. The enzyme does not hydrolyze phosphodiester bonds in single- and double-stranded DNA and RNA.

Applications

• Removal of mRNA before second-strand cDNA synthesis
RT-PCR and qRT-PCR< /a>: Remove RNA after first-strand cDNA synthesis
• Remove the poly(A) sequence of mRNA after hybridization with Oligo(dT)
• Site-specific cleavage of RNA
In vitroResearch on polyadenylation reaction products