Thermo Scientific Ribonuclease I (RNase I) is an endoribonuclease that passes nucleoside 2', The 3'-cyclic monophosphate intermediate preferentially hydrolyzes single-stranded RNA to nucleoside 3'-monophosphate.

The enzyme does not require metal ions to be active.

Product Advantages

• Stable and active under a variety of reaction conditions
• Heat-inactivated within 30 minutes at 100°C< br>
Applications

• Removal of RNA from DNA solutions
• Removal of RNA from recombinant protein preparations
• RNase protection assay
< br>Note

Mammalian ribonuclease inhibitors have no effect on RNase I. RNase I binds to DNA but does not degrade it. The RNase I amino acid sequence is typical of the RNase T2 family. Nonionic detergents such as Triton X-100 do not inhibit RNase I and may even slightly stimulate its activity and stabilize it against thermal inactivation. Triton X-100 or BSA (0.1 mg/mL) prevents RNase I from adhering to glass vessels when working with dilute solutions. Polyamines stimulate RNase I activity.