Thermo Scientific T4 beta-glucosyltransferase (T4 BGT) converts uridine diphosphate glucose (UDP-glucose) The glucose group is transferred to the 5-hydroxymethylcytosine (5-hmC) residue in double-stranded DNA, producing ß-glucosyl-5hydroxymethylcytosine.

Thermo Scientific T4 BGT is formulated for fast reaction times without compromising reaction efficiency. This enzyme can complete 5-hmC glucosylation of 1 μg of DNA in 15 minutes at 37°C.

Key Features

• Specificity—Selectively transfers glucose to the hydroxymethyl group of 5-hmC
• Fast—Glucosylation of 1 μg of DNA in 15 minutes
• Convenient—Comes with optimized buffer and UDP-glucose.

Applications

• Site-specific detection of 5-hmC
• Enrichment of 5-hmC-containing DNA
• Using radioactive UDP- Glucose Donor Labeled 5-hmC Residues

Includes
• T4 β-Glucosyltransferase, 5 U/μL
• 10X Epi Buffer
• 10X UDP-glucose