Thermo Scientific Klenow fragments are large fragments of DNA polymerase I. It exhibits 5'→3' polymerase activity and 3'→5' exonuclease (proofreading) activity, but lacks the 5'→3' exonuclease activity of DNA polymerase I.

Product Advantages

• Incorporation of modified nucleotides (e.g., Cy3, Cy5, aminoallyl, biotin, digoxigenin, and fluorescent Labeled nucleotides)
• Active in restriction enzyme, PCR, RT and T4 ligase buffers

Applications
• DNA by filling in 5'-overhangs Blunt Ending
• Randomly primed DNA labeling
• Labeling by filling in the 5'-overhangs of dsDNA
• DNA sequencing by the Sanger method
• DNA sequencing using synthetic oligonucleotides Site-specific mutagenesis performed
• Second-strand synthesis of cDNA