Glycogen is a branched carbohydrate that is primarily used as a nucleic acid co-precipitate. Packed in 5 test tubes with a capacity of 1 mL, the concentration in each test tube is 5 mg/mL. Features of this glycogen product:
• Ideal for RT-PCR
• Increased precipitation quality
• Quantitative recovery of low concentration (ng/mL) nucleic acids
• Avoid nuclease protection Precipitation loss in experiments
What is a co-precipitant?
Co-precipitating agents are inert substances that aid in nucleic acid recovery during ethanol precipitation. While they can be used to precipitate large amounts of nucleic acid, they are also essential for the quantitative recovery of small amounts of nucleic acid in dilute solutions. Generally, such molecules are used only to reveal the granular precipitate after centrifugation. When used at a final concentration of 50–150 μg/mL, glycogen co-precipitates with nucleic acids in the presence of 0.5 M ammonium acetate and isopropanol or ethanol. It is supplied at a concentration of 5 mg/mL in distilled water treated with diethyl pyrocarbonate. Glycogen does not interfere with 260/280 readings. As with most other preparations of this coprecipitant, this glycogen product is isolated from mussels (biological source). Glycogen is first treated with proteinase K and SDS to remove all contaminating nucleases, then phenol/chloroform extraction, ethanol precipitation, and finally resuspended in nuclease-free water. We guarantee that this glycogen is free of RNase and DNase.
For Research Use Only. Not for use in diagnostic procedures.