Thermo Fisher EP0442 5 x 10,000 units RevertAidreverse transcriptase (200 U/μL)
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EP0442 5 x 10,000 unitsRevertAid reverse transcriptase (200 U/μL)

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Thermo Scientific RevertAid Reverse Transcriptase (RT) is recombinant M-MuLV RT. Its structure and catalytic properties are different from M-MuLV RT. This enzyme not only has RNA-dependent and DNA-dependent polymerase activities, but also has specific RNase H activity on RNA in RNA-DNA hybrids, and its activity is significantly lower than that of avian myeloblastosis virus (AMV) reverse transcriptase .

Product Advantages

•Efficiently synthesize full-length first-strand cDNA up to 13 kb
•Excellent activity at 42°C
• Upper limit activity temperature 50°C
•Can be incorporated with modified nucleotides (such as Cy3, Cy5, rhodamine, aminoallyl, fluorescein-labeled nucleotides)

Application< /b>

•Synthesize first-strand cDNA for RT-PCR and real-time RT-qPCR
•Synthesize cDNA for cloning and expression
•Prepare bands for microarray studies Labeled cDNA Probes
•DNA Labeling
•Analysis of RNA by Primer Extension
For Research Use Only. Not for use in diagnostic procedures.< /div>