< td>

5'		C	C	G	C ↓	G	G		3'
3'			G	G ↑	C	G	C	C		5'

Thermo Scientific Cfr42I (SacII) restriction enzyme recognizes the CCGC^GG site and cleaves best in B buffer at 37°C (isoschizoases: KspI, SacII, Sfr303I, SgrBI, SstII) . For a table of enzymatic activities, double digestion conditions, and heat inactivation of this and other restriction enzymes, see Reaction conditions for restriction enzymes.

Thermo Scientific Conventional Restriction Endonucleases are a large collection of high quality restriction enzymes optimized to function in one buffer of a five-buffer system. In addition, a universal Tango buffer is provided to facilitate double enzyme digestion. All enzymes demonstrated 100% activity under recommended buffers and reaction conditions. To ensure consistent performance, Thermo Scientific Restriction Enzyme Reaction Buffers contain premixed BSA, which enhances the stability of multiple enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—strict quality control and industry-leading production processes
• Convenient color-coded five-buffer system
•Includes universal Tango buffer for double digestion
•Pre-mixed BSA in reaction buffer
•Multiple restriction endonuclease specificities available

< b>Applications

•Molecular cloning
•Restriction site mapping
•Genotyping
•Southern blot
•Restriction fragment length polymorphism ( RFLP)
•SNP

Note:Cfr42I activity is affected by high salt concentrations. Trace amounts of sodium chloride remaining in the substrate DNA after upstream application may inhibit enzyme activity, resulting in incomplete DNA cleavage. Cfr42I, like its prototype SacII, has difficulty cleaving certain sites in lambda DNA. For efficient cleavage, at least 2 copies of the Cfr42I recognition site are required.
See product specifications for information on methylation sensitivity.