Subcloning with Zero Blunt™ TOPO™ PCR Cloning Kit combines unique Zero Background™ technology and pCR™- Blunt II- TOPO™ vectors (Figure 7) combine to allow easy and efficient cloning of blunt-end PCR products.
•Unique technology to reduce background to the lowest possible level
•Enable your cloning – up to 95% efficiency
•Multiple primer sites (T7, T3, M13F, M13R) enable sequence analysis or PCR Assays made simple and convenient
•Kanamycin and Zeocin resistance genes available
•Convenient, validated restriction sites in MCS for further subcloning
•Includes MachI™ T1R competent cells for rapid preparation and shortened cloning time