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Thermo Scientific Bpu10I restriction enzyme recognizes the CCTNAGC(-5/-2)^ site in its unique buffer at 37°C The cutting effect is better. See Reaction Conditions for Restriction Enzymes table to learn about the enzymatic activity of this enzyme and other restriction enzymes , double enzyme digestion conditions and heat inactivation. Note: Also available as FastDigest enzyme for rapid DNA digestion.

Thermo Scientific Conventional Restriction Endonucleases are a large collection of high quality restriction enzymes optimized to function in one buffer of a five-buffer system. Universal Tango buffer is also available to facilitate double digestion. All enzymes demonstrated 100% activity under recommended buffers and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of multiple enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—strict quality control and industry-leading production processes
• Convenient color-coded five-buffer system
•Includes universal Tango buffer for double digestion
•Pre-mixed BSA in reaction buffer
•Multiple restriction endonuclease specificities available

< b>Applications

•Molecular cloning
•Restriction site mapping
•Genotyping
•Southern blot
•Restriction fragment length polymorphism ( RFLP)
•SNP

Note: Too much enzyme (> 4 U/1 µg DNA) may result in incomplete DNA cleavage. Therefore, we recommend extending the incubation time rather than using too much Bpu10I. Star activity may occur with low salt, high glycerol concentrations (>5%), pH > 7.0, or excess enzyme. Cleavage with Bpu10I requires at least two copies of its recognition sequence. For methylation sensitivity, see product specifications.