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5'		T	T ↓	C	G	A	A		3'
3'			A	A	G	C ↑	T	T		5'

Thermo Scientific FastDigest Bsp119I restriction enzyme recognizes the TT^CGAA site and cleaves optimally in 5–15 minutes at 37°C using Universal FastDigest Buffer. Isoschizase: AsuII, Bpu14I, BspT104I, BstBI, Csp45I, NspV, SfuI.

Thermo Scientific FastDigest Bsp119I is one of a series of high-end fast restriction enzymes that are 100% active in both Universal FastDigest Buffer and FastDigest Green Reaction Buffer.

Universal buffer can quickly complete DNA single, double or multiple enzyme digestion in 5–15 minutes without the need for buffer changes or subsequent DNA purification steps. For a table of enzymatic activities, heat inactivation, and incubation times for this and other FastDigest restriction enzymes, see Reaction conditions for FastDigest enzyme< /a>. DNA modifying enzymes (such as Klenow fragment, T4 DNA Ligase, alkaline phosphatase and T4 DNA Polymerase) are 100% active in FastDigest buffer. Therefore, enzymes used in downstream applications can be added directly to the FastDigest reaction mixture.

For convenience, FastDigest Green Buffer contains a density reagent and two tracking dyes for direct gel loading of digestion products.

Universal FastDigest buffer has a short incubation time and an optimal combination of ingredients, thus eliminating the star activity effect.

Features

• All FastDigest enzymes are 100% active in universal buffers
• Buffers are 100% compatible with downstream applications< br>• Complete digestion within 5–15 minutes
• Can be loaded directly for gel electrophoresis after digestion
• No asterisk activity
• 176 FastDigest fast endonuclease provided

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Many restriction fragment lengths Morphology (RFLP)
• SNP Analysis

FastDigest Green Buffer has the same high performance as colorless FastDigest Buffer in DNA digestion reactions and downstream applications. For applications requiring product analysis by fluorescence excitation (eg determination of concentration under UV light), the use of colorless FastDigest buffer is recommended.

See product specifications for information on methylation sensitivity.