Efficiently remove 16S, 23S and 5S rRNA from multiple species
RiboMinus Bacteria 2.0 Transcription The Group Isolation Kit is a complete, automatable kit that efficiently and selectively removes bacterial and archaeal ribosomes from intact total RNA (100 ng to 5 μg) in less than two hours RNA (rRNA).

Key Features of the RiboMinus Bacteria 2.0 Kit:
•High Efficiency—The included RiboMinus pan-prokaryotic probe mix consistently shows less than 10% of reads mapping to 16S and 23S rRNA reads
•Widely used—Use> 150 probe designs targeting> 76 representative bacterial and archaeal species in common phyla and subphyla 16S, 23S and 5S rRNA targets
•Convenient—Kingfisher instruments include Dynabeads MyOne Streptavidin C1 Magnetic Beads and Magnetic Bead Purification Modules and protocols
•Flexible —Can adapt to the quality input of 100 ng to 5 µg total RNA; can handle various total RNA samples with RIN values of 5.0–10.0
•High detection sensitivity—Compared with the previous Increased number of sequencing reads mapped to protein-coding transcripts compared to previous version
•Suitable for metatranscriptomics—removing rRNA from many microbial species in metagenomic samples
< br>Targets rRNA from a wide range of bacteria and archaea
The RiboMinus Bacteria 2.0 kit is based on hybridization capture technology and includes a new RiboMinus pan-prokaryotic probe mix that combines the remaining Ribosomal reads were reduced by 4-fold and showed significant improvements over previous bacterial probe mixtures. The new probe mix utilizes more than 150 3' biotin-labeled ssDNA oligonucleotide designs. These designs were created using highly conserved rRNA regions across 76 pan-prokaryotic species (see table below) and tested with several species (see compatibility list below).

Contains Dynabeads MyOne Streptavidin C1 Purification Kit
Once the probe mixture has selectively hybridized to the target rRNA transcript, it can be purified via the Dynabeads MyOne Capture on streptavidin C1 magnetic beads to complete the removal of the probe-rRNA complex, leaving RNA with rRNA removed. The RiboMinus Bacteria 2.0 kit includes a magnetic bead-based purification module for concentrating rRNA-depleted RNA, making the KingFisher instrument easier to operate, more scalable, and more automated. The resulting rRNA-depleted RNA (RiboMinus RNA) is suitable for whole-transcriptome analysis by RNA-Seq or other downstream applications.

Compatibility
The RiboMinus pan-prokaryote probe mix has been shown to be suitable for the following detection species:
Acinetobacter baumannii
Bacteroides thetaiotaomicron
Bifidobacterium longum infantis subsp.
Campylobacter jejuni
Clostridium difficile
Escherichia coli
Fusobacterium nucleatum i>
Helicobacter pylori
Janssenella
Muellerella thermoacetica
< i>Pseudomonas odorifera
Rickettsia rickettsii
Salmonella typhimurium
Pseudomonas aeruginosa Cyclospora
Salmonella enterica
Staphylococcus aureus
Cavus thermoanaerobacteria< br>Vibrio cholerae
Vibrio fischeri

The RiboMinus pan-prokaryotic probe mix has been demonstrated to be suitable for the following species based on sequence homology :
Corynebacterium
Enterococcus faecalis
Gluconobacter
Myxococcus xanthus
Streptomyces coelicolor