5'			G	G	A	T	C	N4	↓		3'
3'			C	C	T	A	G	N5	↑		5'

Thermo Scientific BspPI (AlwI) restriction enzyme recognizes GGATC(4/5)^ site, buffered in Tango at 55°C The best cleavage effect is in liquid (isocleavage enzymes: AclWI, AlwI). For a table of enzymatic activities, double digestion conditions, and heat inactivation of this and other restriction enzymes, see Reaction conditions for restriction enzymes.

Thermo Scientific Conventional Restriction Endonucleases are a large collection of high quality restriction enzymes optimized to function in one buffer of a five-buffer system. In addition, a universal Tango buffer is provided to facilitate double enzyme digestion. All enzymes demonstrated 100% activity under recommended buffers and reaction conditions. To ensure consistent performance, Thermo Scientific Restriction Enzyme Reaction Buffers contain premixed BSA, which enhances the stability of multiple enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—strict quality control and industry-leading production processes
• Convenient color-coded five-buffer system
•Includes universal Tango buffer for double digestion
•Pre-mixed BSA in reaction buffer
•Multiple restriction endonuclease specificities available

< b>Applications

•Molecular cloning
•Restriction site mapping
•Genotyping
•Southern blot
•Restriction fragment length polymorphism ( RFLP)
•SNP

Notes: 30% activity when incubated at 37°C. Experiments were performed using λ DNA (dam-) (#SD0021). BspPI is blocked by overlapping dam methylation. To avoid dam methylation, use dam-, dcm- strains such as GM2163. See product specifications for methylation sensitivity.