Thermo Scientific 5-fluoroorotic acid (5-fluorouracil-6-carboxylic acid monohydrate; 5-FOA) In the molecular genetics study of yeast, the expression of the URA3 gene (encoding orotate-5'-monophosphate (OMP) dicarboxylase) was detected. Yeast with URA3 gene activity (Ura+) convert 5-FOA into the cytotoxic fluorodeoxyuridine (see figure below). When the medium was supplemented with uracil, only yeast strains with mutations in the URA3 gene could grow in medium containing 5-FOA. 5-FOA is commonly used in experiments with Saccharomyces cerevisiae (URA3), Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3), and Escherichia coli (pyrF).

Applications

•Detection of URA3 gene expression in yeast
•Construction of yeast 2-hybrid library
•Other areas of yeast genetics, including genes Replacement, transposable genetic element analysis and gene mutation identification

Note
•Low solubility in water
•Solubility in dimethyl sulfoxide greater than 150 mg /mL
•Recommend preparation of 100 mg/mL solution (dissolved in dimethyl sulfoxide)