AarI restriction endonuclease can recognize the CACCTGC(4/8)^ site in its specific buffer system (+ oligo) at 37°C for optimal cutting results. Thermo Scientific legacy restriction enzyme products include a broad range of high-quality enzymes optimized for digestion in one of five buffer systems, in addition to the universal Tango buffer provided with the enzymes liquid for double enzyme digestion. All endonucleases are 100% active under recommended buffers and reaction conditions. To ensure stable performance of the endonuclease, Thermo Scientific restriction endonuclease reaction buffers are also premixed with BSA to enhance enzyme stability and bind contaminants that may be present in DNA preparation products.

Features:

• Superior quality—strict quality control and industry-leading production processes
• Five convenient, color-coded buffer systems
• Contains suitable dual-enzyme Universal Tango buffer for cutting
• Reaction buffer pre-mixed with BSA
• Complete range of specific restriction enzymes for a wide selection

Applications< br>• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)< br>

• SNP

Note: See product instructions for methylation sensitivity. To use AarI for cleavage purposes, at least two recognition sites are required. Adding 0.5 μM oligonucleotide containing the AarI recognition sequence to the reaction mixture can significantly improve the DNA cleavage effect, especially for those DNAs with a single AarI site. However, it is difficult to achieve complete cleavage effect with some AarI substrates. More than 10-fold overdigestion with AarI may result in the appearance of star activity. AarI may still bind to cleaved DNA. This characteristic may cause DNA bands to shift during electrophoresis. To avoid atypical thick lines of DNA bands, please prepare samples using 6X DNA loading dye and SDS solution, or heat the digested DNA in the presence of SDS before electrophoresis.