5'

A

C

C

T

G

C

N4

↓

3'

3'

T

G

G

A

C

G

N8

↑

5'

Thermo Scientific BveI (BspMI) restriction enzyme recognizes ACCTGC( 4/8)^ site, cleaves better in O (+oligo) buffer at 37°C (isoschizoases: Acc36I, BfuAI, BspMI). See Reaction Conditions for Restriction Enzymes table to learn about the enzymatic activity of this enzyme and other restriction enzymes , double enzyme digestion conditions and heat inactivation. Note: Also available as FastDigest enzyme for rapid DNA digestion.

Thermo Scientific Conventional Restriction Endonucleases are a large collection of high quality restriction enzymes optimized to function in one buffer of a five-buffer system. In addition, a universal Tango buffer is provided to facilitate double enzyme digestion. All enzymes demonstrated 100% activity under recommended buffers and reaction conditions. To ensure stable endonuclease activity, Thermo Scientific restriction endonuclease reaction buffers are added with BSA, which not only improves the stability of many endonucleases, but also binds to potential contaminants in DNA samples. .

Features

• Superior quality—strict quality control and industry-leading production processes
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for dual degradation
• BSA premixed in reaction buffer
• Broad range of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP

Note: For efficient cleavage, at least two copies of the BveI recognition site are required. Adding 0.5µM oligonucleotide containing BveI recognition sequence to the reaction mixture can significantly improve the digestion efficiency of plasmid DNA, especially DNA with a single BveI site. For some substrates, BveI still has difficulty digesting them completely. The BveI concentration is determined by the maximum cleavage level achieved, and increasing the amount of enzyme does not cause any change in the cleavage profile. Star activity may occur at low salts, high concentrations of glycerol (> 5%), pH values > 8.0, or too much enzyme. BveI may bind to severed DNA. This can cause DNA band drift during electrophoresis. To avoid atypical DNA electrophoresis band patterns, please use 6X DNA loading dye and SDS solution to prepare samples, or add SDS to the DNA digestion product and heat it before electrophoresis. For methylation sensitivity, see product specifications.