Thermo Scientific plasmid pUC57 is a derivative of pUC19 and is 2710 bp in length. pUC57 MCS includes 6 restriction sites with an overhanging 3' end that is resistant toE. coliexonuclease III. This vector is designed for cloning and generation of ExoIII deletions. The exact location of the genetic element (including the stop codon) is shown in the map. DNA replication is initiated at position 890 (± 1) and proceeds in the direction indicated. Nucleotides 2510-2442 (complementary strand) of the bla gene encode a signal peptide.

Product Advantages

• More than 90% supercoiled form
• From E. coli (dam+, i>dcm+)
• Purified by chromatography using proprietary patented technology

Applications

• Cloning
• Insert DNA sequencing

Related products
pUC18 DNA
pUC19 DNA