< td>

5'		C ↓	T	Y	R	A	G		3'
3'			G	A	R	Y	T ↑	C		5'

Thermo Scientific SmoI (SmlI) restriction enzyme recognizes the C^TYRAG site and cleaves well in Tango buffer at 55°C. See Reaction Conditions for Restriction Enzymes table to learn about the enzymatic activity of this enzyme and other restriction enzymes , double enzyme digestion conditions and heat inactivation. Isoschizase: SmlI.

Thermo Scientific Conventional Restriction Endonucleases are a wide range of high-quality restriction enzymes optimized to work in one of the Five Buffer System's buffers. In addition, a universal Tango buffer is provided to facilitate double enzyme digestion. All enzymes demonstrated 100% activity under recommended buffers and reaction conditions. To ensure consistent performance, Thermo Scientific Restriction Enzyme Reaction Buffers contain premixed BSA, which enhances the stability of multiple enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—strict quality control and industry-leading production processes
• Convenient color-coded five-buffer system
•Includes universal Tango buffer for double digestion
•Pre-mixed BSA in reaction buffer
•Multiple restriction endonuclease specificities available

< b>Applications

•Molecular cloning
•Restriction site mapping
•Genotyping
•Southern blot
•Restriction fragment length polymorphism ( RFLP)
•SNP

Note: Activity is 10% when incubated at 37°C. Overlapping dam methylation results in reduced SmoI cleavage. To avoid dam methylation, use dam-, dcm- strains such as GM2163. Star activity may occur at low salt, high concentrations of glycerol (>5%), pH >8.0, or too much enzyme. For methylation sensitivity, see product specifications.