5'

N

N

C

A

S

T

G

N

N ↓

3'< /td>

3'

↑

N

N

G

T

S

A

C

N

N

5'

Thermo Scientific TscAI (TspRI) restriction enzyme recognizes The CASTG(2/-7)^ site cleaves better in Tango buffer at 65°C. See Reaction Conditions for Restriction Enzymes table to learn about the enzymatic activity of this enzyme and other restriction enzymes , double enzyme digestion conditions and heat inactivation. Note: FastDigest enzyme for rapid DNA digestion is also available. Isoschiase: TspRI.

Thermo Scientific Conventional Restriction Endonucleases are a wide range of high-quality restriction enzymes optimized to work in one of the Five Buffer System's buffers. In addition, a universal Tango buffer is provided to facilitate double enzyme digestion. All enzymes demonstrated 100% activity under recommended buffers and reaction conditions. To ensure consistent performance, Thermo Scientific Restriction Enzyme Reaction Buffers contain premixed BSA, which enhances the stability of multiple enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—strict quality control and industry-leading production processes
• Convenient color-coded five-buffer system
•Includes universal Tango buffer for double digestion
•Pre-mixed BSA in reaction buffer
•Multiple restriction endonuclease specificities available

< b>Applications

•Molecular cloning
•Restriction site mapping
•Genotyping
•Southern blot
•Restriction fragment length polymorphism ( RFLP)
•SNP

Note: Activity is less than 5% when incubated at 37°C. Star activity may occur under conditions of low salt, high concentrations of glycerol (>5%), pH values >8.0, or excess enzyme. TscAI generates DNA fragments with a 9-base 3'-extension. To avoid atypical DNA banding patterns, please prepare samples using 6X DNA loading dye and SDS solution, or heat the digested DNA in the presence of SDS before electrophoresis. For methylation sensitivity, see product specifications.